<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Week 6 HW: Genetic Circuits I :: 2026a-elsa-donovan</title><link>https://pages.htgaa.org/2026a/elsa-donovan/homework/week-06-hw-genetic-circuits-part-i/index.html</link><description>DNA Assembly 1. What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose?
According to the New England Biolabs product page, Phusion High-Fidelity PCR Master Mix consists of:
Phusion DNA Polymerase deoxynucleotides a reaction buffer that has been optimized and includes MgCl2 The Phusion DNA polymerase is able to synthesize new DNA strands. It is able to “proofread” itself which means it produces much less errors than other polymerase (hence why it is “high fidelity”). Deoxynucleotides refers to the molecules that makeup DNA (ie. A,T,G,C). These building blocks are going to be used in the new DNA strands. The reaction buffer creates the right environment for this process to take place. It ensure there is the right pH and the right amount of ions.</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/elsa-donovan/homework/week-06-hw-genetic-circuits-part-i/index.xml" rel="self" type="application/rss+xml"/></channel></rss>