https://pages.htgaa.org/2026a/emmanuel-pereyra/homework/week-09-hw-cell-free-systems/index.htmlGeneral homework questions Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell-free expression is more beneficial than cell production. Cell-free protein synthesis offers key advantages over in vivo systems by eliminating the complexity and limitations of the cell as a “black box.” In these systems, all components are defined and manipulable, allowing direct control over variables such as DNA concentrations, expression levels, biochemical composition, cofactors, and reaction conditions. In fact, expression can be precisely adjusted simply by varying the DNA concentration, achieving proportional regulation of each protein—something difficult to achieve in living cells. Furthermore, the system is fully customizable, allowing modification of the internal chemistry and each molecular component, which provides a level of experimental control and predictability far superior to that of traditional cell systems.Hugoen