Week 2 HW: DNA Read, Write, & Edit

Part 1: Benchling & In-silico Gel Art

Above is maybe my favorite Gel Electrophoresis design I created. I was trying to make monster faces, so hopefully this looks sort of like a skull with horns! I made this with the “DNA Gel Art Interface” website created by Ronan at https://rcdonovan.com/gel-art

And below is a screenshot of some of my work in Benchling with the Lambda DNA from https://raw.githubusercontent.com/htgaa/htgaa2023/main/02_gel-art/Lambda_NEB.fasta and restriction enzymes, with the “NEB 2-log” ladder selected in the Virtual Digest tab, and with multiple Digests appearing in the same Virtual Digest, which I reorderdd by dragging the tabs around. These are the same restriction enzymes used in the horned skull drawing at the top of the page.

Part 2: Gel Art - Restriction Digests and Gel Electrophoresis

Below is my DNA gel electrophoresis art looking pretty good after about 45 minutes. This was done at the BUGSS Lab (Baltimore UnderGround Sceince Space) on Sunday, Feb 15. Thanks to Amanda and Joel and everyone else!

Part 3: DNA Design Challenge

Will do in lab on Sunday? Or on my own on Monday?

Part 4: Prepare a Twist DNA Synthesis Order

I created my account, still need to prepare my order …