Labs
Lab writeups:
Week 6 Lab: Gibson Assembly Prelab, use a codon table and convert the following figure to a table of colors of DNA sequences. Variant Bases Original TGTCAG Orange GTTGGA ? Pink GCATGT ? Some photos from lab … Polymerase Chain Reaction (PCR) Above is a photo of our Bio-Rad C1000 PCR Thermal cycler, set for the Backbone Fragment PCR, with Initial Denature: 98 C, 30 seconds, then for 26 Cycles: Denature: 98 C, 10 seconds; Anneal: 57 C, 25 seconds; Extend: 72 C, 1.5 minutes, hen afteer the 26 Cycle: Final Extension: 72 C, 5 minutes; Hold: 12 C, Forever.
Week 7 Lab: Neuromorphic Circuits
Day 1: Write instructions for the OT-2 to execute. I made a few of these, working on getting different results. I tested over 30 of these! I had a few that returned errors! Another design test:
Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell free expression is more beneficial than cell production. Describe the main components of a cell-free expression system and explain the role of each component. Why is energy provision regeneration critical in cell-free systems? Describe a method you could use to ensure continuous ATP supply in your cell-free experiment.
Week 12 Lab: Bioproduction of Beta-Carotene and Lycopene
Questions etc. are here: https://2026a.htgaa.org/2026a/course-pages/weeks/week-12/lab/index.html Post Lab Questions | Mandatory for All Students Which genes when transferred into E. coli will induce the production of lycopene and beta-carotene, respectively? Why do the plasmids that are transferred into the E. coli need to contain an antibiotic resistance gene? What outcomes might we expect to see when we vary the media, presence of fructose, and temperature conditions of the overnight cultures? Generally describe what “OD600” measures and how it can be interpreted in this experiment. OD600 is optical density at 600 nanometer wavelngth … this helps measure how cloudy the liquid is, to determine cell growth.