Week 6 Lab: Gibson Assembly

Week 6 Lab: Gibson Assembly

Prelab, use a codon table and convert the following figure to a table of colors of DNA sequences.

Some photos from lab …

Polymerase Chain Reaction (PCR)

Above is a photo of our Bio-Rad C1000 PCR Thermal cycler, set for the Backbone Fragment PCR, with Initial Denature: 98 C, 30 seconds, then for 26 Cycles: Denature: 98 C, 10 seconds; Anneal: 57 C, 25 seconds; Extend: 72 C, 1.5 minutes, hen afteer the 26 Cycle: Final Extension: 72 C, 5 minutes; Hold: 12 C, Forever.

Making some gel

Above is a photo of Juhi Dhanesha pouring 100ml of TBE for making gel for diagnostic gel electropheresis. Juhi’s htgaa page is at https://pages.htgaa.org/2026a/juhi-dhanesha/

Diagnostic Gel Electrophoresis

Above is a photo from Joel Tyson …