LOCUS       Millikin_SALEM_Construct_Eastey_Quote 7017 bp    DNA     circular UNK 18-APR-2026
DEFINITION  .
ACCESSION   0ebf021d-7532-402c-b61c-0006d81a7dd3
VERSION     0ebf021d-7532-402c-b61c-0006d81a7dd3
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             481..489
                     /color=""
                     /kernel_part_type="cds-no-start-no-stop"
                     /label="BBa_K3183014"
                     /translation="GSG"
     promoter        1..20
                     /color=""
                     /kernel_part_type="promoter"
                     /label="T7 Promoter"
     misc_feature    1186..1232
                     /color=""
                     /kernel_part_type="terminator"
                     /label="T7 Terminator"
     misc_feature    1233..7017
                     /color=""
                     /kernel_part_type="backbone"
                     /label="BBa_K4411019"
     CDS             55..480
                     /color=""
                     /kernel_part_type="cds-yes-start-yes-stop"
                     /label="Millikin Eastey Test 2 Attribution"
                     /translation="MIPETITIQNTQYQVRHQNQVRSNQTFQRMYQWNLIFEFQRIKNQ
                     WIMVSTDIEANDMYAPPQINTEDTIMEISSETIFITPEPQSSIPLENQMQREINNQCEN
                     TPLQQDMAYPESHEDMARYEASTEYERICMILLIKIN*"
     CDS             490..1185
                     /color=""
                     /kernel_part_type="cds-yes-start-yes-stop"
                     /label="mScarlet-I3-NCwt"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGG
                     TVSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARH
                     STGGMDELYK"
     misc_feature    21..54
                     /color=""
                     /kernel_part_type="ribosome-binding-site"
                     /label="A1 RBS"
ORIGIN
        1 taatacgact cactataggg aatgttccct aataatcagc aaagaggtta ctagatgatt
       61 ccggaaacca ttaccattca gaatacccag tatcaggttc gtcatcagaa tcaggttcgt
      121 agcaatcaga cctttcagcg tatgtatcag tggaatctga tttttgaatt tcagcgtatt
      181 aaaaatcagt ggattatggt tagcaccgat attgaagcta atgatatgta tgctccgccg
      241 cagattaata ccgaagatac cattatggaa attagcagcg aaaccatttt tattaccccg
      301 gaaccgcaga gcagcattcc gctggaaaat cagatgcagc gtgaaattaa taatcagtgc
      361 gaaaataccc cgctgcagca ggatatggct tatccggaaa gccatgaaga tatggctcgt
      421 tatgaagcta gcaccgaata tgaacgtatt tgcatgattc tgctgattaa aattaattaa
      481 ggttcaggca tggtgagcaa aggcgaagcg gtgattaaag aatttatgcg ctttaaagtg
      541 catatggaag gcagcatgaa cggccatgaa tttgaaattg aaggcgaagg cgaaggccgc
      601 ccgtatgaag gcacccagac cgcgaaactg aaagtgacca aaggcggccc gctgccgttt
      661 agctgggata ttctgagccc gcagtttatg tatggcagcc gcgcgtttat taaacatccg
      721 gcggatattc cggattattg gaaacagagc tttccggaag gctttaaatg ggaacgcgtg
      781 atgatttttg aagatggcgg caccgtgagc gtgacccagg ataccagcct ggaagatggc
      841 accctgattt ataaagtgaa actgcgcggc ggcaactttc cgccggatgg cccggtgatg
      901 cagaaacgca ccatgggctg ggaagcgagc accgaacgcc tgtatccgga agatgtggtg
      961 ctgaaaggcg atattaaaat ggcgctgcgc ctgaaagatg gcggccgcta tctggcggat
     1021 tttaaaacca cctataaagc gaaaaaaccg gtgcagatgc cgggcgcgtt taacattgat
     1081 cgcaaactgg atattaccag ccataacgaa gattataccg tggtggaaca gtatgaacgc
     1141 agcgtggcgc gccatagcac cggcggcatg gatgaactgt ataaatagca taaccccttg
     1201 gggcctctaa acgggtcttg aggggttttt tgtggcgaat gggacgcgcc ctgtagcggc
     1261 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     1321 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     1381 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     1441 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     1501 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     1561 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     1621 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     1681 atattaacgt ttacaatttc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1741 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgaattaatt cttagaaaaa
     1801 ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt
     1861 ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc
     1921 aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt
     1981 cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg
     2041 tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg
     2101 ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc
     2161 gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg
     2221 gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa
     2281 tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt
     2341 acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac
     2401 catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg
     2461 cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg
     2521 agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca
     2581 agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga
     2641 cagttttatt gttcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2701 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2761 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2821 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     2881 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2941 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3001 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3061 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3121 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3181 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3241 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3301 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3361 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3421 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3481 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     3541 tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     3601 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     3661 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     3721 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     3781 aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt
     3841 tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag
     3901 cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga
     3961 tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt
     4021 actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg
     4081 atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat
     4141 gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg
     4201 cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat
     4261 gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc
     4321 ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac
     4381 aggagcacga tcatgcgcac ccgtggggcc gccatgccgg cgataatggc ctgcttctcg
     4441 ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg
     4501 aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa
     4561 atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata
     4621 agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct
     4681 ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg
     4741 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     4801 ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac
     4861 cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa
     4921 gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg
     4981 gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac
     5041 gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac
     5101 cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga
     5161 catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata
     5221 tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag
     5281 cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc
     5341 atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg
     5401 aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat
     5461 gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac
     5521 gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt
     5581 aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat
     5641 cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc
     5701 cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac
     5761 cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac
     5821 tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg
     5881 aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct
     5941 gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg
     6001 gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca
     6061 cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt
     6121 cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc
     6181 cggcgtagag gatcgagatc tcgatcccgc gaaattcaag aggatccgac ttctttacac
     6241 tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag
     6301 gagctaagga agctaaataa tacgactcac tataggggaa ttgtgagcgg ataacaattc
     6361 ccctcaagaa ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc
     6421 atcatcatca tcacagcagc ggcctggtgc cgcgcggcag ccatatggct agcatgactg
     6481 gtggacagca aatgggtcgc ggttccgagt tcgagctccg tcgacaagct tgcatgacca
     6541 tgattacgat tgccaagctt gaatccactg gccgtcgttt tacaacgcgc agtttaaggt
     6601 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat
     6661 tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa
     6721 agtcccccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac
     6781 caccgatatg gccagtgtgc cggtatccgt tatcggggaa gaagtggctg atctcagcca
     6841 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aagatccggc
     6901 tgctaacaaa gcccgaaaga tccggatata gttcctcctt tcagctagca taaccccttg
     6961 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggat
//
