Week 13 HW: Synbio

Final Project Build

This week has been focused on key milestones for my final project.

• BioLight V5 circular plasmid finalized
• Benchling construct submitted to the Node Order Form as approved
• TWIST order simulated with no errors reported
• pUC19 confirmed as vector with availabiliy at Genspace
• Scheduled a visit to Genspace on May 28th to attend Safety Training and Orientation
• Aim 1 objective is to validate viability of the Clonal Gene “BioLight V5” to be used in the “Photoplasm” optogenetic labware
• Slides for final project are drafted
• ChimeraX being used to generate Mechanism of Action illustrations.
• pDawn with sfGFP identified and ordered from AddGene as a control to enable testing of protocols
• Design, Development, and physical prototyping (Build) of the “Photoplasm” device is proceeding, with sensor data.
• Additional applications developed (software) to facilitate electrical engineering schemats.
• All steps being documented as an open-source build framework, Aim 2 objective in support of the larger Aim 3 “MakerSpace” vision. Reviewed and contributed to the “Ai Tutor” project led by @Derek - Shared a Q&A prompt based on accuracy & confidence in a feedback loop.