https://pages.htgaa.org/2026a/fabrizio-flores-huaman/homework/week-09-hw-cell-free-systems/index.htmlThis week introduces synthesis of proteins using cellular machinery outside of a cell. Homework Homework Part A: General and Lecturer-Specific Questions General homework questions Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell-free expression is more beneficial than cell production. The biggest advantage of cell-free systems is that they offer an open environment where you have total control over experimental variables like pH and salt concentrations without a cell membrane getting in the way. This flexibility is especially beneficial when producing antimicrobial peptides or lysis proteins that would normally kill a living host, as well as for high-throughput screening of genetic circuits where you need to test many DNA variants in hours rather than waiting days for cultures to grow.Hugoen