https://pages.htgaa.org/2026a/fatima-fragoso-hernandez/homework/week-06-hw-genetic-circuits-part-i/index.htmlDNA ASSEMBLY Phusion High-Fidelity PCR Master Mix Components Phusion HF PCR Master Mix is provided as a 2X stock that is diluted to a 1X concentration in the final reaction. While the sources do not list every chemical ingredient, they indicate its purpose is to amplify specific DNA sequences (such as the amilCP gene and the mUAV backbone) with high accuracy. Standard high-fidelity master mixes like Phusion typically contain: Phusion DNA Polymerase: A highly accurate enzyme with proofreading activity to minimize mutations. dNTPs (Deoxynucleotide Triphosphates): The building blocks (A, T, C, G) used to synthesize the new DNA strand. Buffer: Maintains the optimal pH and ionic strength for the enzyme. $MgCl_2$: A necessary cofactor for the DNA polymerase to function. Factors Determining Primer Annealing TemperatureHugoen