https://pages.htgaa.org/2026a/fiona-connolly/homework/week-06-hw-genetic-circuits-part-i/index.htmlWeek 6 — Genetic Circuits I: Assembly Technologies Part 1 — DNA Assembly: PCR, Gibson, Golden Gate, and transformation A topic guide on the molecular-biology toolkit that underpins all of synthetic biology: amplifying DNA (PCR), cutting it (restriction enzymes), joining it (Gibson and Golden Gate), and getting it into cells (transformation). Written as a stand-alone primer rather than a homework Q&A. Part 2 (Asimov Kernel: building genetic circuits computationally) will follow as a separate page once the simulation work is complete.Hugoen