https://pages.htgaa.org/2026a/flavoris-belue/homework/week-06-genetic-circuits-part-i/index.htmlQuestions 1. What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Phusion DNA Polymerase: Catalyzes the synthesis of new DNA strands. Has 3′→5′ exonuclease proofreading activity, which removes incorrectly added nucleotides. Phusion polymerase is a genetically engineered DNA polymerase fused to a DNA-binding domain. The fusion domain increases DNA binding, which improves processivity. Reaction buffer: Help to maintain a stable pH. Also provides optimal ionic strength for polymerase activity. Stabilizes enzyme structure at high temperatures. Magnesium Chloride (MgCl₂): Essential cofactor for DNA polymerases. Coordinates with the phosphate groups of incoming nucleotides. Helps stabilize primer–template interactions. dNTPs: Provide the substrates used to synthesize new DNA strands. Each nucleotide carries three phosphates, providing the energy needed for polymerization. 2. What are some factors that determine primer annealing temperature during PCR?Hugoen