https://pages.htgaa.org/2026a/flavoris-belue/homework/week-10-advanced-imaging-and-measurement-technology/index.htmlHomework: Final Project For your final project: Please identify at least one (ideally many) aspect(s) of your project that you will measure. It could be the mass or sequence of a protein, the presence, absence, or quantity of a biomarker, etc. For Aim 1 of my final project, there are several things I’ll need to measure, from confirming the construct is correct, to confirming the cells are expressing it, to ultimately quantifying the light output that defines success or failure of the experiment. The most important measurement is luminescence intensity from the IPTG-induced cells co-expressing nnLuz v4 truncated and nnH3H v2 after hispidin supplementation. This is the readout that directly tests my hypothesis that the v4 mutations stacked with the truncation produce a brighter enzyme pair than either modification alone. Light output alone isn’t enough without knowing the cells are actually doing what I think they’re doing, so I’ll also measure cell density (OD600) and perform a colony PCR to confirm the insert is present in transformed colonies.Hugoen