https://pages.htgaa.org/2026a/flo-razoux/homework/week_02_hw/dna_construct_order/index.htmlDesign 01 : Substitution within Template Following the steps demonstrated during recitation Week 02, I cut the sfGFP insert from the plasmid template ColE1-AmpR-sfGFP and replaced it with the optimized firefly luciferase sequence (including flanks 5’ and 3’). Step 01: Load plasmid template ColE1-AmpR-sfGFP in Benchling Step 02: Copy plasmid template ColE1-AmpR-sfGFP into a new project page, cut sfGFP and paste the optimized luciferase sequence.Hugoen