Lab Automation

1.Python Script for Opentrons Artwork

Generation of a glowing uterus in GUI

HOW IT STARTED cover image cover image

HOW IT’S GOING cover image cover image


DOCUMENTATION

(1) Searching for “uterus image vector” on the web (2) Loading best image (simple graphic design and good color contrast) in Automation Art Interface (3) Adjusting Zoom (0.9), Brightness (70%), Contrast (110%) Pixelation (-10%) and Saturation (300%) (4) Refining design manually (incl. testing different colorations) (5) Exporting coordinates: mscarlet_i_points = [(-7.7, 20.9),(-5.5, 20.9),(-3.3, 20.9),(-1.1, 20.9),(1.1, 20.9),(3.3, 20.9),(5.5, 20.9),(7.7, 20.9),(-14.3, 18.7),(-12.1, 18.7),>(-9.9, 18.7),(-7.7, 18.7),(7.7, 18.7),(9.9, 18.7),(12.1, 18.7),(14.3, 18.7),(-29.7, 16.5),(-27.5, 16.5),(-25.3, 16.5),(-23.1, 16.5),(-20.9, 16.5),(-18.7, 16.5),(-16.5, 16.5),(-14.3, 16.5),(-12.1, 16.5),(-5.5, 16.5),(-3.3, 16.5),(-1.1, 16.5),(1.1, 16.5),(3.3, 16.5),(5.5, 16.5),(12.1, 16.5),(14.3, 16.5),(16.5, 16.5),(18.7, 16.5),(20.9, 16.5),(23.1, 16.5),(25.3, 16.5),(27.5, 16.5),(29.7, 16.5),(-31.9, 14.3),(-29.7, 14.3),(-27.5, 14.3),(-25.3, 14.3),(-23.1, 14.3),(-20.9, 14.3),(-18.7, 14.3),(-16.5, 14.3),(-9.9, 14.3),(-7.7, 14.3),(-5.5, 14.3),(-3.3, 14.3),(-1.1, 14.3),(1.1, 14.3),(3.3, 14.3),(5.5, 14.3),(7.7, 14.3),(9.9, 14.3),(16.5, 14.3),(18.7, 14.3),(20.9, 14.3),(23.1, 14.3),(25.3, 14.3),(27.5, 14.3),(29.7, 14.3),(31.9, 14.3),(-34.1, 12.1),(-31.9, 12.1),(-29.7, 12.1),(-16.5, 12.1),(-14.3, 12.1),(-12.1, 12.1),(-9.9, 12.1),(-7.7, 12.1),(-5.5, 12.1),(-3.3, 12.1),(-1.1, 12.1),(1.1, 12.1),(3.3, 12.1),(5.5, 12.1),(7.7, 12.1),(9.9, 12.1),(12.1, 12.1),(16.5, 12.1),(29.7, 12.1),(31.9, 12.1),(34.1, 12.1),(-34.1, 9.9),(-29.7, 9.9),(-14.3, 9.9),(-12.1, 9.9),(-9.9, 9.9),(-7.7, 9.9),(-5.5, 9.9),(-3.3, 9.9),(-1.1, 9.9),(1.1, 9.9),(3.3, 9.9),(5.5, 9.9),(7.7, 9.9),(9.9, 9.9),(14.3, 9.9),(29.7, 9.9),(34.1, 9.9),(-34.1, 7.7),(-29.7, 7.7),(-27.5, 7.7),(-25.3, 7.7),(-14.3, 7.7),(-12.1, 7.7),(-9.9, 7.7),(-7.7, 7.7),(-5.5, 7.7),(-3.3, 7.7),(-1.1, 7.7),(1.1, 7.7),(3.3, 7.7),(5.5, 7.7),(7.7, 7.7),(12.1, 7.7),(14.3, 7.7),(25.3, 7.7),(27.5, 7.7),(34.1, 7.7),(-34.1, 5.5),(-23.1, 5.5),(-12.1, 5.5),(-9.9, 5.5),(-7.7, 5.5),(-5.5, 5.5),(-3.3, 5.5),(-1.1, 5.5),(1.1, 5.5),(3.3, 5.5),(5.5, 5.5),(7.7, 5.5),(12.1, 5.5),(23.1, 5.5),(34.1, 5.5),(-34.1, 3.3),(-23.1, 3.3),(-20.9, 3.3),(-12.1, 3.3),(-9.9, 3.3),(-7.7, 3.3),(-5.5, 3.3),(-3.3, 3.3),(-1.1, 3.3),(1.1, 3.3),(3.3, 3.3),(5.5, 3.3),(12.1, 3.3),(20.9, 3.3),(23.1, 3.3),(34.1, 3.3),(-31.9, 1.1),(-18.7, 1.1),(-12.1, 1.1),(-9.9, 1.1),(-7.7, 1.1),(-5.5, 1.1),(-3.3, 1.1),(-1.1, 1.1),(1.1, 1.1),(3.3, 1.1),(5.5, 1.1),(9.9, 1.1),(12.1, 1.1),(18.7, 1.1),(31.9, 1.1),(-31.9, -1.1),(-18.7, -1.1),(-9.9, -1.1),(-7.7, -1.1),(-5.5, -1.1),(-3.3, -1.1),(-1.1, -1.1),(1.1, -1.1),(3.3, -1.1),(9.9, -1.1),(18.7, -1.1),(31.9, -1.1),(-29.7, -3.3),(-27.5, -3.3),(-20.9, -3.3),(-9.9, -3.3),(-7.7, -3.3),(-5.5, -3.3),(-3.3, -3.3),(-1.1, -3.3),(1.1, -3.3),(3.3, -3.3),(7.7, -3.3),(9.9, -3.3),(20.9, -3.3),(27.5, -3.3),(29.7, -3.3),(-25.3, -5.5),(-23.1, -5.5),(-7.7, -5.5),(-5.5, -5.5),(-3.3, -5.5),(-1.1, -5.5),(1.1, -5.5),(3.3, -5.5),(7.7, -5.5),(23.1, -5.5),(25.3, -5.5),(-7.7, -7.7),(-5.5, -7.7),(-3.3, -7.7),(-1.1, -7.7),(1.1, -7.7),(7.7, -7.7),(-7.7, -9.9),(-3.3, -9.9),(-1.1, -9.9),(1.1, -9.9),(7.7, -9.9),(-7.7, -12.1),(-3.3, -12.1),(-1.1, -12.1),(1.1, -12.1),(3.3, -12.1),(7.7, -12.1),(-7.7, -14.3),(-5.5, -14.3),(5.5, -14.3),(7.7, -14.3),(-5.5, -16.5),(5.5, -16.5),(-5.5, -18.7),(-3.3, -18.7),(3.3, -18.7),(5.5, -18.7),(-5.5, -20.9),(-3.3, -20.9),(3.3, -20.9),(5.5, -20.9),(-5.5, -23.1),(-3.3, -23.1),(3.3, -23.1),(5.5, -23.1),(-5.5, -25.3),(-3.3, -25.3),(3.3, -25.3),(5.5, -25.3),(-5.5, -27.5),(-3.3, -27.5),(3.3, -27.5),(5.5, -27.5),(-5.5, -29.7),(-3.3, -29.7),(3.3, -29.7),(5.5, -29.7)] mko2_points = [(14.3, 12.1),(12.1, 9.9),(-31.9, 7.7),(9.9, 7.7),(31.9, 7.7),(-29.7, 5.5),(9.9, 5.5),(29.7, 5.5),(-29.7, 3.3),(-27.5, 3.3),(-25.3, 3.3),(7.7, 3.3),(9.9, 3.3),(25.3, 3.3),(27.5, 3.3),(29.7, 3.3),(-29.7, 1.1),(-27.5, 1.1),(-25.3, 1.1),(-23.1, 1.1),(-20.9, 1.1),(7.7, 1.1),(20.9, 1.1),(23.1, 1.1),(25.3, 1.1),(27.5, 1.1),(29.7, 1.1),(-27.5, -1.1),(-25.3, -1.1),(-23.1, -1.1),(5.5, -1.1),(7.7, -1.1),(23.1, -1.1),(25.3, -1.1),(27.5, -1.1),(5.5, -3.3),(5.5, -5.5),(3.3, -7.7),(5.5, -7.7),(3.3, -9.9)] mrfp1_points = [(-5.5, 18.7),(-3.3, 18.7),(-1.1, 18.7),(1.1, 18.7),(3.3, 18.7),(5.5, 18.7),(-9.9, 16.5),(-7.7, 16.5),(7.7, 16.5),(9.9, 16.5),(-14.3, 14.3),(-12.1, 14.3),(12.1, 14.3),(14.3, 14.3),(-31.9, 9.9),(31.9, 9.9),(29.7, 7.7),(-31.9, 5.5),(-27.5, 5.5),(-25.3, 5.5),(25.3, 5.5),(27.5, 5.5),(31.9, 5.5),(-31.9, 3.3),(31.9, 3.3),(-29.7, -1.1),(-20.9, -1.1),(20.9, -1.1),(29.7, -1.1),(-25.3, -3.3),(-23.1, -3.3),(23.1, -3.3),(25.3, -3.3),(-5.5, -9.9),(5.5, -9.9),(-5.5, -12.1),(5.5, -12.1),(-3.3, -14.3),(-1.1, -14.3),(1.1, -14.3),(3.3, -14.3),(-3.3, -16.5),(-1.1, -16.5),(1.1, -16.5),(3.3, -16.5),(-1.1, -18.7),(1.1, -18.7),(-1.1, -20.9),(1.1, -20.9),(-1.1, -23.1),(1.1, -23.1),(-1.1, -25.3),(1.1, -25.3),(-1.1, -27.5),(1.1, -27.5)]

(6) Laptop/internet crashed before submission for publication in the gallery 😢

HTGAA26 Opentrons Colab Textbook

HOW IT STARTED cover image cover image

HOW IT’S GOING cover image cover image


DOCUMENTATION ChatGPT was used to (1) understand code lines from the CoLab examples, (2) understand and fix errors when trying to write my own code from the CoLab examples, (3) and later when trying to adapt the code downloaded from Ronan Donovan’s AAI. Step (2) and (3) were not successful despite many attempts. At last, documentation of other students were checked and the code of the design Golden Lyre by Katherine Kolin was used as a base. The code was easily adapted and the first test images were generated in Colab quickly after.

Glowing Uterus Colab Code

Finally, coloring tests were made to get as close as possible of the fluorescent images published in the gallery

cover image cover image

2.Post-Lab Questions

To be updated on March 10th