https://pages.htgaa.org/2026a/flo-razoux/homework/week_06_hw/index.htmlHugoenhttps://pages.htgaa.org/2026a/flo-razoux/homework/week_06_hw/dna-assembly/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026a/flo-razoux/homework/week_06_hw/dna-assembly/index.htmlTHE CHROMOPHORE COLOR CLONING QUEST Lab Protocol 1. Components of the Phusion High-Fidelity PCR Master Mix The Phusion HF PCR Master Mix is a ready-to-use “all-in-one” cocktail designed for DNA amplification. It contains: Phusion DNA polymerase: copy the DNA with robust performance and high accuracy (error rate much lower than common polymerase such as Taq). Deoxynucleotides (dNTPs): free A, T, C and G building blocks needed to copy new DNA strands. Reaction Buffer: an optimized chemical buffer that keeps the PCR environment stable and optimal for the DNA polymerase to copy DNA with the highest accuracy level. MgCl2: salts needed for the polymerase to function. What needs to be added before running the PCR: template, primers and water.https://pages.htgaa.org/2026a/flo-razoux/homework/week_06_hw/gibson-assembly-benchling/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026a/flo-razoux/homework/week_06_hw/gibson-assembly-benchling/index.html