https://pages.htgaa.org/2026a/flo-razoux/homework/week_11_hw/cell_free_reagents/index.html1. Composition and role of the different reagents BL21 (DE3) Star Lysate is a ready-to-use cell extract from E. coli that contains all the machinery needed to carry out transcription and translation processes, such as ribosomes, tRNAs, and enzymes etc. The star refers to a mutation that reduces the activity of the RNase E and thus, protects the system from mRNA degradation. This lysate also contains a special enzyme, the T7 RNA polymerase. This enzyme recognizes the T7 promoter and thus enables it to focus the transcription on target genes in a more efficient way than E. coli’s own polymerase.Hugoen