https://pages.htgaa.org/2026a/flo-razoux/homework/week_11_hw/master_mix_design/index.html1. Properties of the fluorescent proteins In cell-free systems, each fluorescent protein differs in how quickly it folds and matures, how bright its signal is, and how sensitive it is to conditions like oxygen or pH. sfGFP matures very quickly (~13.6 minutes) and folds reliably even under difficult conditions or when fused to other proteins, giving a fast and strong fluorescence readout that closely tracks protein production. However, it still requires oxygen for chromophore formation, which can limit fluorescence in low-oxygen environments.Hugoen