https://pages.htgaa.org/2026a/florian-kroh/homework/week-09-hw-cell-free-systems/index.htmlPart A: General and Lecturer-Specific Questions General homework questions Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell-free expression is more beneficial than cell production. Cell-free protein synthesis provides major advantages over traditional in vivo protein expression because the reaction occurs outside living cells, giving researchers direct control over reaction conditions and components. Variables such as DNA concentration, salts, energy substrates, cofactors, temperature, and additives can be adjusted independently without needing to maintain cell viability, allowing rapid optimization and faster experimental iteration. Another key advantage is flexibility: proteins can be expressed immediately after adding DNA templates, without time-consuming cloning, transformation, or cell culturing steps. Cell-free systems also allow incorporation of non-natural amino acids, toxic proteins, or synthetic circuits that would otherwise harm or interfere with living cells. Two important cases where cell-free expression is more beneficial than cell-based production are:Hugoen