https://pages.htgaa.org/2026a/francisco-martinez/homework/week-09-hw-cell-free-systems/index.htmlHomework Part A: General and Lecturer-Specific Questions Advantages: Cell-free systems allow direct control of reaction conditions without maintaining cell viability. They are especially useful for toxic proteins and membrane proteins. Main components: Cell extract or Tx/Tl machinery, DNA template, amino acids, nucleotides, energy source, salts, and buffer. Together, these support transcription, translation, and reaction stability. Energy regeneration: ATP and GTP are rapidly consumed during transcription and translation. Continuous supply can be maintained with phosphoenolpyruvate- or maltodextrin-based regeneration systems.Hugoen