https://pages.htgaa.org/2026a/franziska-matt/homework/week-06-hw-genetic-circuits-part-i/index.htmlDNA Assembly Answer these questions about the protocol in this week’s lab: What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Typical components include: Phusion DNA Polymerase A high-fidelity enzyme that synthesizes new DNA strands with very low error rates (proofreading activity → fewer mutations). dNTPs (deoxynucleotide triphosphates) Building blocks (A, T, G, C) used to create new DNA strands. Reaction Buffer (HF buffer) Maintains optimal pH and salt conditions for enzyme activity and fidelity. Mg²⁺ ions (magnesium chloride) Essential cofactor for polymerase activity; affects enzyme efficiency and specificity. Stabilizers (sometimes included) Help maintain enzyme stability during thermal cycling. What are some factors that determine primer annealing temperature during PCR?Hugoen