<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Week 6 HW: Genetic Circuits Part I :: 2026a-gabriela-frajtag</title><link>https://pages.htgaa.org/2026a/gabriela-frajtag/homework/week-06-hw-genetic-circuits-part-i/index.html</link><description>Assignment: DNA Assembly 1. What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? The Phusion High-Fidelity PCR Master Mix contains Phusion DNA polymerase, a enzyme that synthesizes DNA (this one also has proofreading activity). It also includes HF buffer, which provides the optimal pH and salt concentration (MgCl2) required for enzyme activity, as well as dNTPs, which are the nucleotide building blocks used to synthesize the new DNA strands. Glycerol helps stabilize the enzyme, and DMSO may be added to improve amplification of GC-rich regions by reducing secondary structure formation.</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/gabriela-frajtag/homework/week-06-hw-genetic-circuits-part-i/index.xml" rel="self" type="application/rss+xml"/></channel></rss>