https://pages.htgaa.org/2026a/gabriela-frajtag/homework/week-09-hw-cell-free-systems/index.htmlGeneral homework questions 1. Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell-free expression is more beneficial than cell production. Cell-free protein synthesis (CFPS) offers much greater flexibility and experimental control than in vivo expression because protein production occurs outside living cells, typically in a test tube using cellular extracts. This means variables such as DNA concentration, ion composition, temperature, cofactors, chaperones, and energy substrates can be precisely adjusted without worrying about cell viability, metabolism, or toxicity. Another notable example is time, as to perform CFPS takes 1 –2 days, whereas in vivo protein expression may take 1–2 weeks. Here are two examples of when CFPS can be efficiently used:Hugoen