Labs
Lab writeups:
Benchling Gel Design Restriction Digest Protocol Gel Preparation Protocol Gel Run and Imaging Protocol Final Gel Image
Opentrons Design Opentrons CoLab Code ### Green cursor = center_location.move(types.Point(x=-18, y = 12)) pipette_20ul.pick_up_tip() pipette_20ul.aspirate(15, location_of_color('Green')) dispense_and_detach(pipette_20ul, 3, cursor) cursor = cursor.move(types.Point(x=-4, y=4)) for i in range(4): dispense_and_detach(pipette_20ul, 3, cursor) if i!=3: cursor = cursor.move(types.Point(y=4)) pipette_20ul.aspirate(15, location_of_color('Green')) for i in range(2): cursor = cursor.move(types.Point(x=4, y=4)) dispense_and_detach(pipette_20ul, 3, cursor) cursor = cursor.move(types.Point(x=4)) for i in range(3): dispense_and_detach(pipette_20ul, 3, cursor) if i!=2: cursor = cursor.move(types.Point(x=4, y=-4)) pipette_20ul.aspirate(18, location_of_color('Green')) cursor = cursor.move(types.Point(x=4)) for i in range(3): dispense_and_detach(pipette_20ul, 3, cursor) if i!=2: cursor = cursor.move(types.Point(x=4, y=4)) cursor = cursor.move(types.Point(x=4)) for i in range(3): dispense_and_detach(pipette_20ul, 3, cursor) if i!=2: cursor = cursor.move(types.Point(x=4, y=-4)) pipette_20ul.aspirate(15, location_of_color('Green')) cursor = cursor.move(types.Point(y=-4)) for i in range(2): dispense_and_detach(pipette_20ul, 3, cursor) if i!=1: cursor = cursor.move(types.Point(y=-4)) cursor = cursor.move(types.Point(y=-4)) for i in range(3): dispense_and_detach(pipette_20ul, 3, cursor) if i!=2: cursor = cursor.move(types.Point(x=-4, y=-4)) cursor = cursor.move(types.Point(x=-4)) pipette_20ul.aspirate(18, location_of_color('Green')) for i in range(6): dispense_and_detach(pipette_20ul, 3, cursor) if i!=5: cursor = cursor.move(types.Point(x=-4)) cursor = cursor.move(types.Point(x=-4)) pipette_20ul.aspirate(15, location_of_color('Green')) for i in range(3): dispense_and_detach(pipette_20ul, 3, cursor) if i!=2: cursor = cursor.move(types.Point(x=-4, y=-4)) cursor = cursor.move(types.Point(y=-4)) for i in range(2): dispense_and_detach(pipette_20ul, 3, cursor) if i!=1: cursor = cursor.move(types.Point(y=-4)) cursor = cursor.move(types.Point(y=-4)) pipette_20ul.aspirate(15, location_of_color('Green')) for i in range(3): dispense_and_detach(pipette_20ul, 3, cursor) if i!=2: cursor = cursor.move(types.Point(x=4, y=-4)) cursor = cursor.move(types.Point(x=-12, y=16)) for i in range(2): dispense_and_detach(pipette_20ul, 3, cursor) if i!=1: cursor = cursor.move(types.Point(x=-4)) cursor = cursor.move(types.Point(x=-4, y=-4)) pipette_20ul.aspirate(9, location_of_color('Green')) dispense_and_detach(pipette_20ul, 3, cursor) cursor = cursor.move(types.Point(y=-4)) for i in range(2): dispense_and_detach(pipette_20ul, 3, cursor) if i!=1: cursor = cursor.move(types.Point(x=4, y=-4)) cursor = cursor.move(types.Point(x=-4, y=-4)) pipette_20ul.aspirate(12, location_of_color('Green')) for i in range(4): dispense_and_detach(pipette_20ul, 3, cursor) if i!=3: cursor = cursor.move(types.Point(x=4)) cursor = cursor.move(types.Point(x=12)) pipette_20ul.aspirate(18, location_of_color('Green')) for i in range(6): dispense_and_detach(pipette_20ul, 3, cursor) if i!=5: cursor = cursor.move(types.Point(x=4)) cursor = cursor.move(types.Point(x=4)) pipette_20ul.aspirate(15, location_of_color('Green')) for i in range(3): dispense_and_detach(pipette_20ul, 3, cursor) if i!=2: cursor = cursor.move(types.Point(x=4, y=4)) cursor = cursor.move(types.Point(y=4)) for i in range(2): dispense_and_detach(pipette_20ul, 3, cursor) if i!=1: cursor = cursor.move(types.Point(y=4)) cursor = cursor.move(types.Point(y=4)) pipette_20ul.aspirate(15, location_of_color('Green')) for i in range(2): dispense_and_detach(pipette_20ul, 3, cursor) if i!=1: cursor = cursor.move(types.Point(x=-4, y=4)) cursor = cursor.move(types.Point(x=8, y=-8)) dispense_and_detach(pipette_20ul, 3, cursor) cursor = cursor.move(types.Point(x=4)) for i in range(2): dispense_and_detach(pipette_20ul, 3, cursor) if i!=1: cursor = cursor.move(types.Point(x=4, y=-4)) cursor = cursor.move(types.Point(y=-4)) pipette_20ul.aspirate(18, location_of_color('Green')) for i in range(2): dispense_and_detach(pipette_20ul, 3, cursor) if i!=1: cursor = cursor.move(types.Point(x=-4, y=-4)) cursor = cursor.move(types.Point(x=4, y=-4)) for i in range(4): dispense_and_detach(pipette_20ul, 3, cursor) if i!=3: cursor = cursor.move(types.Point(x=-4)) pipette_20ul.drop_tip() pipette_20ul.pick_up_tip() ### Red cursor = cursor.move(types.Point(x=-8, y=44)) pipette_20ul.aspirate(12, location_of_color('Red')) for i in range(2): dispense_and_detach(pipette_20ul, 3, cursor) if i!=1: cursor = cursor.move(types.Point(x=-4)) cursor = cursor.move(types.Point(y=4)) for i in range(2): dispense_and_detach(pipette_20ul, 3, cursor) if i!=1: cursor = cursor.move(types.Point(x=4)) cursor = cursor.move(types.Point(x=-24)) pipette_20ul.aspirate(12, location_of_color('Red')) for i in range(2): dispense_and_detach(pipette_20ul, 3, cursor) if i!=1: cursor = cursor.move(types.Point(x=-4)) cursor = cursor.move(types.Point(y=-4)) for i in range(2): dispense_and_detach(pipette_20ul, 3, cursor) if i!=1: cursor = cursor.move(types.Point(x=4)) cursor = cursor.move(types.Point(x=4, y=-8)) pipette_20ul.aspirate(18, location_of_color('Red')) for i in range(4): dispense_and_detach(pipette_20ul, 3, cursor) if i!=3: cursor = cursor.move(types.Point(x=4)) cursor = cursor.move(types.Point(x=-4, y=-4)) for i in range(2): dispense_and_detach(pipette_20ul, 3, cursor) if i!=1: cursor = cursor.move(types.Point(x=-4)) pipette_20ul.drop_tip()