https://pages.htgaa.org/2026a/hadjer-begga/projects/individual-final-project/phase-6-twist-order-simulation/index.htmlTwist Bioscience Order Simulation: After completing the design and optimization of all fragments in Benchling , I exported the finalized sequences in FASTA format. Each fragment corresponded to a specific part of the structural or maturation multicassette constructs and already contained the required overlaps for Gibson Assembly. I then uploaded the FASTA files into the Twist Bioscience Platform using the gene synthesis workflow. The platform automatically analyzed each sequence to evaluate synthesis compatibility, including repetitive regions, GC balance, and sequence complexity.Hugoen