<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Phase 8: Full Construct Assembly (insert into pCAMBIA1300 and pCAMBIA2300) :: 2026a-hadjer-begga</title><link>https://pages.htgaa.org/2026a/hadjer-begga/projects/individual-final-project/phase-8-full-construct-assembly-insert-into-pcambia1300-and-pcambia2300-/index.html</link><description>Full Construct Assembly: After successfully assembling the Structural and Maturation multicassette inserts in Phase 7, I moved to the final cloning step where I inserted each complete multicassette block into its corresponding binary vector backbone using in silico Gibson Assembly in Benchling.
For this phase, I followed the same general Gibson Assembly workflow previously used for multicassette reconstruction. However, instead of assembling several independent fragments together, I assembled only two major components: the linearized pCAMBIA vector backbone and the complete multicassette insert.</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/hadjer-begga/projects/individual-final-project/phase-8-full-construct-assembly-insert-into-pcambia1300-and-pcambia2300-/index.xml" rel="self" type="application/rss+xml"/></channel></rss>