from opentrons import types

metadata = {    # see https://docs.opentrons.com/v2/tutorial.html#tutorial-metadata
    'author': 'Heather Qian',
    'protocolName': 'Opentrons Flower',
    'description': 'Makes a red and green flower',
    'source': 'HTGAA 2026 Opentrons Lab',
    'apiLevel': '2.20'
}

##############################################################################
###   Robot deck setup constants - don't change these
##############################################################################

TIP_RACK_DECK_SLOT = 9
COLORS_DECK_SLOT = 6
AGAR_DECK_SLOT = 5
PIPETTE_STARTING_TIP_WELL = 'A1'

well_colors = {
    'A1' : 'Red',
    'B1' : 'Green',
    'C1' : 'Orange'
}


def run(protocol):
  ##############################################################################
  ###   Load labware, modules and pipettes
  ##############################################################################

  # Tips
  tips_20ul = protocol.load_labware('opentrons_96_tiprack_20ul', TIP_RACK_DECK_SLOT, 'Opentrons 20uL Tips')

  # Pipettes
  pipette_20ul = protocol.load_instrument("p20_single_gen2", "right", [tips_20ul])

  # Modules
  temperature_module = protocol.load_module('temperature module gen2', COLORS_DECK_SLOT)

  # Temperature Module Plate
  temperature_plate = temperature_module.load_labware('opentrons_96_aluminumblock_generic_pcr_strip_200ul',
                                                      'Cold Plate')
  # Choose where to take the colors from
  color_plate = temperature_plate

  # Agar Plate
  agar_plate = protocol.load_labware('htgaa_agar_plate', AGAR_DECK_SLOT, 'Agar Plate')  ## TA MUST CALIBRATE EACH PLATE!
  # Get the top-center of the plate, make sure the plate was calibrated before running this
  center_location = agar_plate['A1'].top()

  pipette_20ul.starting_tip = tips_20ul.well(PIPETTE_STARTING_TIP_WELL)

  ##############################################################################
  ###   Patterning
  ##############################################################################

  ###
  ### Helper functions for this lab
  ###

  # pass this e.g. 'Red' and get back a Location which can be passed to aspirate()
  def location_of_color(color_string):
    for well,color in well_colors.items():
      if color.lower() == color_string.lower():
        return color_plate[well]
    raise ValueError(f"No well found with color {color_string}")

  # For this lab, instead of calling pipette.dispense(1, loc) use this: dispense_and_detach(pipette, 1, loc)
  def dispense_and_detach(pipette, volume, location):
      """
      Move laterally 5mm above the plate (to avoid smearing a drop); then drop down to the plate,
      dispense, move back up 5mm to detach drop, and stay high to be ready for next lateral move.
      5mm because a 4uL drop is 2mm diameter; and a 2deg tilt in the agar pour is >3mm difference across a plate.
      """
      assert(isinstance(volume, (int, float)))
      above_location = location.move(types.Point(z=location.point.z + 5))  # 5mm above
      pipette.move_to(above_location)       # Go to 5mm above the dispensing location
      pipette.dispense(volume, location)    # Go straight downwards and dispense
      pipette.move_to(above_location)       # Go straight up to detach drop and stay high

  ###
  ### YOUR CODE HERE to create your design
  ###

  # Center
  pipette_20ul.pick_up_tip()
  pipette_20ul.aspirate(18, location_of_color("Green"))

  cursor = center_location.move(types.Point(x=-4, y=15))

  for i in range(9):
    dispense_and_detach(pipette_20ul, 3, cursor)
    if i != 8:
      cursor = cursor.move(types.Point(x=4))
    if i % 3 == 2 and i != 8:
      cursor = cursor.move(types.Point(x=-12, y=-4))
    if i == 5: 
      pipette_20ul.aspirate(9, location_of_color("Green"))

  pipette_20ul.drop_tip()

  # Right petal
  pipette_20ul.pick_up_tip()
  pipette_20ul.aspirate(18, location_of_color("Red"))

  cursor = cursor.move(types.Point(x=4))

  for i in range(6):
    dispense_and_detach(pipette_20ul, 3, cursor)
    cursor = cursor.move(types.Point(y=4))
    if i % 3 == 2:
      cursor = cursor.move(types.Point(x=4, y=-12))

  pipette_20ul.aspirate(3, location_of_color("Red"))
  cursor = cursor.move(types.Point(y=4))
  dispense_and_detach(pipette_20ul, 3, cursor)

  # Top petal
  pipette_20ul.aspirate(18, location_of_color("Red"))

  cursor = cursor.move(types.Point(x=-12, y=8))

  for i in range(6):
    dispense_and_detach(pipette_20ul, 3, cursor)
    cursor = cursor.move(types.Point(x=-4))
    if i % 3 == 2:
      cursor = cursor.move(types.Point(x=12, y=4))

  pipette_20ul.aspirate(3, location_of_color("Red"))
  cursor = cursor.move(types.Point(x=-4))
  dispense_and_detach(pipette_20ul, 3, cursor)

  # Left petal
  pipette_20ul.aspirate(18, location_of_color("Red"))

  cursor = cursor.move(types.Point(x=-8, y=-12))

  for i in range(6):
    dispense_and_detach(pipette_20ul, 3, cursor)
    cursor = cursor.move(types.Point(y=-4))
    if i % 3 == 2:
      cursor = cursor.move(types.Point(x=-4, y=12))

  pipette_20ul.aspirate(3, location_of_color("Red"))
  cursor = cursor.move(types.Point(y=-4))
  dispense_and_detach(pipette_20ul, 3, cursor)

  # Bottom petal
  pipette_20ul.aspirate(18, location_of_color("Red"))

  cursor = cursor.move(types.Point(x=12, y=-8))
  
  for i in range(6):
    dispense_and_detach(pipette_20ul, 3, cursor)
    cursor = cursor.move(types.Point(x=4))
    if i % 3 == 2:
      cursor = cursor.move(types.Point(x=-12, y=-4))

  pipette_20ul.aspirate(3, location_of_color("Red"))
  cursor = cursor.move(types.Point(x=4))
  dispense_and_detach(pipette_20ul, 3, cursor)

  pipette_20ul.drop_tip()

  # Stem
  pipette_20ul.pick_up_tip()
  pipette_20ul.aspirate(18, location_of_color("Green"))
  
  for i in range(6):
    cursor = cursor.move(types.Point(y=-4))
    dispense_and_detach(pipette_20ul, 3, cursor)

  # Right leaf
  pipette_20ul.aspirate(15, location_of_color("Green"))

  cursor = cursor.move(types.Point(x=4, y=8))

  for i in range(5):
    dispense_and_detach(pipette_20ul, 3, cursor)
    if i % 2 == 0:
      cursor = cursor.move(types.Point(y=4))
    else:
      cursor = cursor.move(types.Point(x=4))

  # Left leaf
  pipette_20ul.aspirate(15, location_of_color("Green"))

  cursor = cursor.move(types.Point(x=-16, y=-12))

  for i in range(5):
    dispense_and_detach(pipette_20ul, 3, cursor)
    if i % 2 == 0:
      cursor = cursor.move(types.Point(y=4))
    else:
      cursor = cursor.move(types.Point(x=-4))

  pipette_20ul.drop_tip()