Abstract Project Aims Aim 1: Assemble the antirepressor circuit using Gibson Assembly. Parts will include the putative antirepressors from Extracellular Prophage-Inducing Particles (EPIPs), an inducible promoter, a fluorescent protein, and plasmid backbone. Then, electroporate the plasmid into Mycolicibacterium aichiense, and use fluorescence to evaluate the success of transformation and gene expression. Investigate the effect of putative antirepressors on M. aichiense growth by tracking OD600 over time.