https://pages.htgaa.org/2026a/henrietta-scholtz/homework/week-06-hw-genetic-circuits-part-i/index.htmlDNA Assembly What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? The Phusion HF PCR Master Mix contains several key components that are already pre-combined for convenience. Phusion DNA Polymerase is the core enzyme responsible for copying the DNA template, and it has a built-in proofreader to ensure it is high-fidelity, meaning it reduces errors during amplification. The dNTPs provide the nucleotide building blocks that get incorporated into the new DNA strand. MgCl₂ (magnesium) acts as an essential cofactor that activates the polymerase. The reaction buffer (oven conditions in my analogy) maintains the correct pH and ionic environment for the reaction to work. For this particular lab, precise mutagenesis of the amilCP chromophore region was required, so the high-fidelity polymerase is especially important; it ensures there are no unintended amino acid changes beyond the designed mutation.Hugoen