Labs

Lab writeups:

  • Week 1 Lab: Pipetting

  • Week 2 Lab: Gel Art

  • Week 3 Lab: Opentrons

    Python Script for Opentrons Artwork Since I am not present to interact directly with the Opentrons output, I thought about why I would want to pipette an image and what that image should represent and decided to use Ndebele bead patterns as inspiration. Ndebele bead patterns have a very specific geometric logic. They are built on a grid of “bead units” arranged in bold, angular, symmetric designs. The traditional South Ndebele aesthetic uses high-contrast colors in step-like diagonal and horizontal bands, often with thick outlines and mirrored symmetry.

  • Week 4 Lab: Protein Part I

    *Part of Week 4 Homework/See above

  • Week 5 Lab: Protein Part II

    *Part of Week 5 Homework/See above

  • Week 6 Lab: Gibson Assembly

    Lab questions answered on the Week 6 Homework page.

  • Week 7 Lab: Neuromorphic Circuits

    Weiss Lab run of my IANNS biased dependent parallel AND Gate (This was not really the HW question I’m just facinated by AND Gates and did not think mine would be run as we a large cohort, but not many submitted). The results do seem to point to my parrallel expression working for a smooth release of slightly differently weighted levels. Every dot is a human cell! Neuromorphic Wizard test that shows both parts are rising and one slight weighted less and also see what could be ‘drift’

  • Week 09 Lab: Cell Free

    LAB QUESTIONS CELL FREE I answered these in relation to lab document and FP where relevent. Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell free expression is more beneficial than cell production. Cell-free systems allow direct control over reaction components without needing viable cells, making them ideal for expressing toxic proteins (like AMPs) and for rapidly iterating fusion protein designs like CBM27_RGD_MaSp1. Specifically chosen to bypass the living cells.