https://pages.htgaa.org/2026a/henrietta-scholtz/projects/individual-final-project/additional/index.htmlDraft v 1 Experimental Protocol: CBM27_RGD_MaSp1_4x Fusion Expression and Tremella Composite Formation Construct: CBM27_RGD_MaSp1_4x_Fusion Vector: pET28a Expression system: NEB PURExpress E6800 Protein MW: ~32 kDa Step 1: Cell-Free Expression Materials needed: PURExpress Solution A PURExpress Solution B Murine RNase Inhibitor Template DNA (linear PCR product or circular plasmid, 250 ng) Nuclease-free water Ice Protocol:Hugoen