<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Week 6 HW: Genetic Circuits Part I :: 2026a-ian-teran</title><link>https://pages.htgaa.org/2026a/ian-teran/homework/week-06-hw-genetic-circuits-part-i/index.html</link><description>Assignment: DNA Assembly
Answer these questions about the protocol in this week’s lab:
1. What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose?
The Phusion High-Fidelity PCR Master Mix contains several key components necessary for efficient and accurate DNA amplification. First, it includes Phusion DNA polymerase, a high-fidelity enzyme with proofreading activity (3’ → 5’ exonuclease), which reduces errors during DNA replication. It also contains dNTPs (deoxynucleotide triphosphates), which are the building blocks used to synthesize new DNA strands. The mix includes a reaction buffer, optimized with the correct pH and salt concentrations to ensure proper enzyme activity. Additionally, it contains Mg²⁺ ions, which act as essential cofactors for the polymerase. Some mixes may also include stabilizers to maintain enzyme activity during thermal cycling.</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/ian-teran/homework/week-06-hw-genetic-circuits-part-i/index.xml" rel="self" type="application/rss+xml"/></channel></rss>