https://pages.htgaa.org/2026a/ian-teran/homework/week-09-hw-cell-free-systems/index.htmlHomework Part A: General and Lecturer-Specific Questions General homework questions 1. Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell-free expression is more beneficial than cell production. Cell-free protein synthesis (CFPS) offers significant advantages over traditional in vivo expression systems, primarily due to its flexibility and precise control over experimental conditions. Because CFPS operates in an open environment without living cells, researchers can directly manipulate the concentrations of DNA templates, ions, cofactors, and other components in real time. This eliminates constraints associated with cellular viability, such as toxicity or metabolic burden. As a result, CFPS is particularly advantageous for the production of proteins that are toxic to host cells, such as antimicrobial peptides or pore-forming proteins. Additionally, CFPS enables rapid prototyping of genetic constructs, making it highly suitable for applications like synthetic biology circuit testing, where speed and iterative design are essential.Hugoen