https://pages.htgaa.org/2026a/iman-karibzhanova/homework/week-06-hw-genetic-circuits-part-i/index.htmlAssignment: DNA Assembly What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Components included are Phusion DNA Polymerase, deoxynucleotides (dNTPs), and HF Reaction Buffer containing MgCl₂. Physion DNA polymerase is a Pyrococcus-like enzyme. The polymerase domain reads the strand and synthesizes it by adding dNTPs one at a time (through phosphodeister bond formation). This requires a primer to already be present in the template. The enhancing domain fused onto the polymerase keeps the enzyme attached to the DNA template, speeding up the process. The exonuclease domain reads this newly synthesized stand and corrects any mistakes. Deoxynucleotides (dNTPs) are the building blocks of DNA at optimized concentrations. The HF reaction buffer helps control the pH for optimal activity, while the MgCl₂ positions the dNTPs correctly and stabilizes the transition state during bond formation.Hugoen