HTGAA 2026: Individual Final Project Documentation SECTION 1: ABSTRACT My project aims to engineer an autonomous, glowing plant using the firefly light-producing system. If successful, it will be the first demonstration of an autonomously glowing plant host through the firefly-based pathway. The significance of this work lies in several values, including cross-kingdom metabolic engineering and the longstanding challenge of solving the missing steps in the luciferin bioluminescence pathway. More broadly, this project asks the question of whether a plant can be rewired to support sustained luciferase-based bioluminescence.