Homework
Weekly homework submissions:
Week 1 HW: Principles and Practices
Important Resources Week 1 Lecture Recording Week 1 Recitation Recording Week 1 Recitation Slides Engineered bacterial cellulose to make self-pigmenting, living textiles. I have chosen to explore a bioengineering application for creating more sustainable systems in the textile industry and more personally, an alternative material for use in design and the visual arts. Specifically, I am analysing the proposal of engineered bacterial cellulose to produce self-pigmenting textiles. In this analysis, I focus on preventing environmental harm, ensuring bio-safety, and equitable production in the deployment of this technology. I also briefly extend the discussion to the ethical implications of engineered bacterial cellulose for other bio-fabrication applications, such as increasing material yield, creating sensing and responsive materials, or affecting its mechanical structure, all applications that I am intrigued by and are seeds for potential final projects. Walker, K. T., Li, I. S., Keane, J., Goosens, V. J., Song, W., Lee, K.-Y., & Ellis, T. (2025). Self-pigmenting textiles grown from cellulose-producing bacteria with engineered tyrosinase expression. Nature Biotechnology, 43(3), 345–354. https://doi.org/10.1038/s41587-024-02194-3. Image from online article: https://www.nature.com/articles/s41587-024-02194-3
Week 2 HW: DNA Read, Write and Edit
Important Resources George Church lecture Joe Jacobson lecture Emily Leproust Lecture Part 1: Benchling & In-silico Gel Art Using Benchling, I imported the Lambda DNA and began simulating the Restriction Enzyme Digestion with the following enzymes: EcoRI-HF, HindIII-HF, BamHI-HF, KpnI-HF, EcoRV-HF, SacI-HF, SalI-HF.
Important Resources Recitation Recording Recitation Slides Lab Overview FINAL PROJECT IDEAS: 3 Initial Proposals. Final Project Ideas by Isobel Leonard Homework Part 1: Python Script for Opentrons Artwork This week we are creating a Python file to run on an Opentrons OT-2 liquid handling robot to create flourescent designs. This is achieved by depositing E.coli genetically engineered to express different fluorescent proteins onto black agar plates. The flouresence will be visible under UV light and so will our designs!