Labs
Lab writeups:
Week 5; Phage Lysis Protein Design Challenge
Phage Lysis Protein Design Challenge L-Protein Engineering (Mutagenesis) Designing these mutants with good computational confidence is hard. It will show you limitations of some of the structure based models. Ultimately, you can pick various combinations of mutations and get lab results and then decide to pick the next round of mutations, but this assay will not be easy to run at scale in this class. L PROTEIN SEQ:
Overview | Background In this two-day lab, you will design and build your very own IANN using a library of plasmids from the Ron Weiss lab and human embryonic kidney (HEK) 293 cells. IANNs differ from traditional synthetic genetic circuits because IANNs can perform analog computations, rather than being limited to digital computations. IANNs are also universal function approximators–given an adequate number of intracellular artificial neurons, you can use an IANN to achieve any input/output behavior you’d like.
Week 12: Bioproduction of Beta-Carotene and Lycopene
Post Lab Questions | Mandatory for All Students Which genes when transferred into E. coli will induce the production of lycopene and beta-carotene, respectively? Why do the plasmids that are transferred into the E. coli need to contain an antibiotic resistance gene? What outcomes might we expect to see when we vary the media, presence of fructose, and temperature conditions of the overnight cultures?