Week 1 Lab: Introduction to Pipetting and Dilutions

Overview

    • Date(s): 03/02/26 – 03/03/26
    • Notes: Reviewed lab materials outlined in ‘Overview’ protocol section (pipette types and tips, tubes, tube holders, and stock reagents) and concentration basics with Kate Carline (William & Mary Node TA). Discussed lab material functions and reviewed the basics of dilution math and pipetting technique.
    • Supporting Picture(s):
      • Pipetting_Tuesday_10 Pipetting_Tuesday_10

Part 1: Mixing Color

  • Prepared tubes with red, yellow, and blue food coloring solution
  • Marked 6 tubes with red, yellow, blue, red/yellow, blue/yellow, and red/blue combinations
  • Added 500 uL to each each red, yellow, blue, red/yellow, blue/yellow, and red/blue combination solution tube
  • See above – made combinations by mixing colors
  • See above
  • See above
  • Dispersed concentrations onto wax paper to make design in lieu of petri plate

    • Supporting Part 1 and Part 2 photos below

      Pipetting_Mon_1 Pipetting_Mon_1Pipetting_Tuesday_1 Pipetting_Tuesday_1Pipetting_Tuesday_2 Pipetting_Tuesday_2Pipetting_Tuesday_3 Pipetting_Tuesday_3Pipetting_Tuesday_4 Pipetting_Tuesday_4Pipetting_Tuesday_5 Pipetting_Tuesday_5Pipetting_Tuesday_6 Pipetting_Tuesday_6Pipetting_Tuesday_7 Pipetting_Tuesday_7Pipetting_Tuesday_8 Pipetting_Tuesday_8Pipetting_Tuesday_9 Pipetting_Tuesday_9

      Practiced basic pipetting, mixing colors, and performing serial dilution

Part 2: Performing Serial Dilution

  • Performed serial dilutions on MS/food coloring
  • Made a final serial dilution reaction based on the information in the pre-lab
    • See pictures above