https://pages.htgaa.org/2026a/jean-colmenares/homework/week-06-hw-genetic-circuits-part-i/index.htmlPART 1: Protocol questions What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? The mastermix contains: Phusion Hi-Fi DNA Polymerase: It is crucial for completing the amplicons generated during PCR. Deoxynucleotides: The building blocks necessary for replicating DNA fragments. Buffer including MgCl2: Prevents enzyme denaturation by maintaining pH at a fixed level. What are some factors that determine primer annealing temperature during PCR? Some factors include the primer lengthHugoen