https://pages.htgaa.org/2026a/jenn-leung/labs/week-07-cell-free-systems/index.htmlProtein synthesis requires transcription and translation Transcription eukaryotes or in cytoplasm in prokaryotes RNA polymerase DNA nucleotides to make RNA polymerase will bind promoter and in the space will Translation tRNA, amino acids and ribosomes, mRNA inside nucleas, splicing take introns and leave extrons RBS (ribosome binding site) - attach to small subunit - mRNA to merge with small subunit tRNA will bind start codon (AUG/ ATG) (complementary to start codon) codons are 3 nucleotides EPA E P A are three sites of tRNA working on codons [exit, peptide, amino acid] bind to start, peptides growing protein on P site, then amino acid on site and leave ribosomeHugoen