Wet-lab:
In-person run protocols Remote Opentrons based Gingko Nebula Cloud Competent e.coli Cloning transformation kit Plating media (LB) Antibiotic selection marker
Adhesion protein binding peptide / cardiac junction protein
PC12 - enhance dopaminic thing or find disease what would cells secret with dopamine? How to do read out?
Synaptic dopamine release is positively regulated by SNAP-25 that involves in benzo[a]pyrene-induced neurotoxicity
https://www.sciencedirect.com/science/article/abs/pii/S0045653519315991?via%3Dihub this paper confirms that DRD1 and DRD3 are endogenously expressed in PC12 and co-localise with SNAP-25, and that CRISPR-Cas9 plasmid transfection works effectively in these cells using Lipofectamine 2000 — directly relevant to your BSL-1 modification approach. The SNAP-25/DRD interaction also suggests that if you’re overexpressing DRD1 for your reinforcement system, SNAP-25 expression levels will influence how effectively that receptor gets trafficked