LOCUS       PETase in pET blank (KAN)                5850 bp DNA     circular   17-FEB-2026
DEFINITION                               PETase in pET blank (KAN)
COMMENT                                  Resistance markers: Kanamycin

COMMENT                                  This file was created using tools provided by Twist Bioscience
FEATURES                                 Location/Qualifiers
     source                              1..5850
                                         /organism="PETase in pET blank (KAN)"
     misc_feature_                       1..870
                                         /label="insert"
     misc_feature_                       871..5850
                                         /label="vector_backbone-870-5850"
     misc_feature_                       1041..1347
                                         /label="f1"
     misc_feature_                       1572..2387
                                         /label="kan"
     misc_feature_                       2509..3097
                                         /label="colE1_high_copy"
     misc_feature_                       3527..3718
                                         /label="ROP"
     misc_feature_                       4256..4277
                                         /label="CAP_binding"
     misc_feature_                       4290..5381
                                         /label="lacI"
     misc_feature_                       5373..5450
                                         /label="lacIQ_promoter"
     misc_feature_                       5763..5781
                                         /label="T7"
     misc_feature_                       5781..5808
                                         /label="lacO"
     misc_feature_                       5821..5843
                                         /label="RBS_3"
     misc_feature_                       5826..5842
                                         /label="T7_transl_en_RBS"
ORIGIN
        1 ATGAACTTCC CGCGCGCCAG CCGCCTGATG CAGGCCGCCG TGCTGGGCGG CCTGATGGCC
       61 GTGAGCGCCG CCGCCACCGC CCAGACCAAC CCGTACGCCC GCGGCCCGAA CCCGACCGCC
      121 GCCAGCCTGG AGGCCAGCGC CGGCCCGTTC ACCGTGCGCA GCTTCACCGT GAGCCGCCCG
      181 AGCGGCTACG GCGCCGGCAC CGTGTACTAC CCGACCAACG CCGGCGGCAC CGTGGGCGCC
      241 ATCGCCATCG TGCCGGGCTA CACCGCCCGC CAGAGCAGCA TCAAGTGGTG GGGCCCGCGC
      301 CTGGCCAGCC ACGGCTTCGT GGTGATCACC ATCGACACCA ACAGCACCCT GGACCAGCCG
      361 AGCAGCCGCA GCAGCCAGCA GATGGCCGCC CTGCGCCAGG TGGCCAGCCT GAACGGCACC
      421 AGCAGCAGCC CGATCTACGG CAAGGTGGAC ACCGCCCGCA TGGGCGTGAT GGGCTGGAGC
      481 ATGGGCGGCG GCGGCAGCCT GATCAGCGCC GCCAACAACC CGAGCCTGAA GGCCGCCGCC
      541 CCGCAGGCCC CGTGGGACAG CAGCACCAAC TTCAGCAGCG TGACCGTGCC GACCCTGATC
      601 TTCGCCTGCG AGAACGACAG CATCGCCCCG GTGAACAGCA GCGCCCTGCC GATCTACGAC
      661 AGCATGAGCC GCAACGCCAA GCAGTTCCTG GAGATCAACG GCGGCAGCCA CAGCTGCGCC
      721 AACAGCGGCA ACAGCAACCA GGCCCTGATC GGCAAGAAGG GCGTGGCCTG GATGAAGCGC
      781 TTCATGGACA ACGACACCCG CTACAGCACC TTCGCCTGCG AGAACCCGAA CAGCACCCGC
      841 GTGAGCGACT TCCGCACCGC CAACTGCAGC TTAATACCTA GGCTGCTAAA CAAAGCCCGA
      901 AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
      961 TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATTGGCGAAT
     1021 GGGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA
     1081 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG
     1141 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT
     1201 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG
     1261 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA
     1321 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT
     1381 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT
     1441 TTAACGCGAA TTTTAACAAA ATATTAACGT TTACAATTTC AGGTGGCACT TTTCGGGGAA
     1501 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA
     1561 TGAATTAATT CTTAGAAAAA CTCATCGAGC ATCAAATGAA ACTGCAATTT ATTCATATCA
     1621 GGATTATCAA TACCATATTT TTGAAAAAGC CGTTTCTGTA ATGAAGGAGA AAACTCACCG
     1681 AGGCAGTTCC ATAGGATGGC AAGATCCTGG TATCGGTCTG CGATTCCGAC TCGTCCAACA
     1741 TCAATACAAC CTATTAATTT CCCCTCGTCA AAAATAAGGT TATCAAGTGA GAAATCACCA
     1801 TGAGTGACGA CTGAATCCGG TGAGAATGGC AAAAGTTTAT GCATTTCTTT CCAGACTTGT
     1861 TCAACAGGCC AGCCATTACG CTCGTCATCA AAATCACTCG CATCAACCAA ACCGTTATTC
     1921 ATTCGTGATT GCGCCTGAGC GAGACGAAAT ACGCGATCGC TGTTAAAAGG ACAATTACAA
     1981 ACAGGAATCG AATGCAACCG GCGCAGGAAC ACTGCCAGCG CATCAACAAT ATTTTCACCT
     2041 GAATCAGGAT ATTCTTCTAA TACCTGGAAT GCTGTTTTCC CGGGGATCGC AGTGGTGAGT
     2101 AACCATGCAT CATCAGGAGT ACGGATAAAA TGCTTGATGG TCGGAAGAGG CATAAATTCC
     2161 GTCAGCCAGT TTAGTCTGAC CATCTCATCT GTAACATCAT TGGCAACGCT ACCTTTGCCA
     2221 TGTTTCAGAA ACAACTCTGG CGCATCGGGC TTCCCATACA ATCGATAGAT TGTCGCACCT
     2281 GATTGCCCGA CATTATCGCG AGCCCATTTA TACCCATATA AATCAGCATC CATGTTGGAA
     2341 TTTAATCGCG GCCTAGAGCA AGACGTTTCC CGTTGAATAT GGCTCATAAC ACCCCTTGTA
     2401 TTACTGTTTA TGTAAGCAGA CAGTTTTATT GTTCATGACC AAAATCCCTT AACGTGAGTT
     2461 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT
     2521 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG
     2581 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA
     2641 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT
     2701 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA
     2761 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC
     2821 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT
     2881 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA
     2941 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG
     3001 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT
     3061 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT
     3121 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA
     3181 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC
     3241 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT
     3301 CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC
     3361 TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG
     3421 CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG
     3481 CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC
     3541 CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG
     3601 ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA
     3661 ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG
     3721 ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA
     3781 GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG
     3841 GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC
     3901 AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC
     3961 AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC
     4021 GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC
     4081 TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC
     4141 CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCTAGT CATGCCCCGC GCCCACCGGA
     4201 AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG AGATCCCGGT GCCTAATGAG
     4261 TGAGCTAACT TACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT
     4321 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC
     4381 GCCAGGGTGG TTTTTCTTTT CACCAGTGAG ACGGGCAACA GCTGATTGCC CTTCACCGCC
     4441 TGGCCCTGAG AGAGTTGCAG CAAGCGGTCC ACGCTGGTTT GCCCCAGCAG GCGAAAATCC
     4501 TGTTTGATGG TGGTTAACGG CGGGATATAA CATGAGCTGT CTTCGGTATC GTCGTATCCC
     4561 ACTACCGAGA TGTCCGCACC AACGCGCAGC CCGGACTCGG TAATGGCGCG CATTGCGCCC
     4621 AGCGCCATCT GATCGTTGGC AACCAGCATC GCAGTGGGAA CGATGCCCTC ATTCAGCATT
     4681 TGCATGGTTT GTTGAAAACC GGACATGGCA CTCCAGTCGC CTTCCCGTTC CGCTATCGGC
     4741 TGAATTTGAT TGCGAGTGAG ATATTTATGC CAGCCAGCCA GACGCAGACG CGCCGAGACA
     4801 GAACTTAATG GGCCCGCTAA CAGCGCGATT TGCTGGTGAC CCAATGCGAC CAGATGCTCC
     4861 ACGCCCAGTC GCGTACCGTC TTCATGGGAG AAAATAATAC TGTTGATGGG TGTCTGGTCA
     4921 GAGACATCAA GAAATAACGC CGGAACATTA GTGCAGGCAG CTTCCACAGC AATGGCATCC
     4981 TGGTCATCCA GCGGATAGTT AATGATCAGC CCACTGACGC GTTGCGCGAG AAGATTGTGC
     5041 ACCGCCGCTT TACAGGCTTC GACGCCGCTT CGTTCTACCA TCGACACCAC CACGCTGGCA
     5101 CCCAGTTGAT CGGCGCGAGA TTTAATCGCC GCGACAATTT GCGACGGCGC GTGCAGGGCC
     5161 AGACTGGAGG TGGCAACGCC AATCAGCAAC GACTGTTTGC CCGCCAGTTG TTGTGCCACG
     5221 CGGTTGGGAA TGTAATTCAG CTCCGCCATC GCCGCTTCCA CTTTTTCCCG CGTTTTCGCA
     5281 GAAACGTGGC TGGCCTGGTT CACCACGCGG GAAACGGTCT GATAAGAGAC ACCGGCATAC
     5341 TCTGCGACAT CGTATAACGT TACTGGTTTC ACATTCACCA CCCTGAATTG ACTCTCTTCC
     5401 GGGCGCTATC ATGCCATACC GCGAAAGGTT TTGCGCCATT CGATGGTGTC CGGGATCTCG
     5461 ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT
     5521 GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC
     5581 CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC
     5641 CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC
     5701 GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCGATCTCG ATCCCGCGAA
     5761 ATTAATACGA CTCACTATAG GGGAATTGTG AGCGGATAAC AATTCCCCTC TAGAAATAAT
     5821 TTTGTTTAAC TTTAAGAAGG AGATATACAT
//