LOCUS       phaC_expression_constru 1977 bp ds-DNA     linear       27-FEB-2026
DEFINITION  .
COMMENT     C. necator phaC gene, codon optimized for E coli. Added promoter,
            RBS, His-tag, and terminator for full expression cassette.  labeled
            as phaC_Ec in Twist synthesis order (not actually ordered, just
            getting a quote)
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label="Promoter_BBa_J23106"
                     /note="https://parts.igem.org/Part:BBa_J23106"
     RBS             36..57
                     /label="RBS_BBa_B0034"
                     /note="https://parts.igem.org/Part:BBa_B0034"
     CDS             58..1824
                     /label="phaC CDS (poly-beta-hydroxybuterate polymerase)"
     gene            58..1824
                     /label="phaC gene"
     CDS             58..1848
                     /label="PhaC"
                     /note="(Cn)CO-Ec"
                     /translation="MATGKGAAASTQEGKSQPFKVTPGPFDPATWLEWSRQWQGTEGNGHAAASGIPGLDALAGVKIAPAQLGDIQQRYMKDFSALWQAMAEGKAEATGPLHDRRFAGDAWRTNLPYRFAAAFYLLNARALTELADAVEADAKTRQRIRFAISQWVDAMSPANFLATNPEAQRLLIESGGESLRAGVRNMMEDLTRGKISQTDESAFEVGRNVAVTEGAVVFENEYFQLLQYKPLTDKVHARPLLMVPPCINKYYILDLQPESSLVRHVVEQGHTVFLVSWRNPDASMAGSTWDDYIEHAAIRAIEVARDISGQDKINVLGFCVGGTIVSTALAVLAARGEHPAASVTLLTTLLDFADTGILDVFVDEGHVQLREATLGGGAGAPCALLRGLELANTFSFLRPNDLVWNYVVDNYLKGNTPVPFDLLFWNGDATNLPGPWYCWYLRHTYLQNELKVPGKLTVCGVPVDLASIDVPTYIYGSREDHIVPWTAAYASTALLANKLRFVLGASGHIAGVINPPAKNKRSHWTNDALPESPQQWLAGAIEHHGSWWPDWTAWLAGQAGAKRAAPANYGNARYRAIEPAPGRYVKAKAHHHHHHH*"
     misc_feature    1825..1845
                     /label="7xHis-tag"
     misc_feature    1846..1848
                     /label="stop codon"
     terminator      1849..1977
                     /label="Terminator_BBa_B0015"
                     /note="https://parts.igem.org/Part:BBa_B0015"
ORIGIN
        1 TTTACGGCTA GCTCAGTCCT AGGTATAGTG CTAGCCATTA AAGAGGAGAA AGGTACCatg
       61 gcgaccggca aaggcgcggc ggcgagcacc caggaaggca aaagccagcc gtttaaagtg
      121 accccgggcc cgtttgatcc ggcgacctgg ctggaatgga gccgccagtg gcagggcacc
      181 gaaggcaacg gccatgcggc ggcgagcggc attccgggcc tggatgcgct ggcgggcgtg
      241 aaaattgcgc cggcgcagct gggcgatatt cagcagcgct atatgaaaga ttttagcgcg
      301 ctgtggcagg cgatggcgga aggcaaagcg gaagcgaccg gcccgctgca tgatcgccgc
      361 tttgcgggcg atgcgtggcg caccaacctg ccgtatcgct ttgcggcggc gttttatctg
      421 ctgaacgcgc gcgcgctgac cgaactggcg gatgcggtgg aagcggatgc gaaaacccgc
      481 cagcgcattc gctttgcgat tagccagtgg gtggatgcga tgagcccggc gaactttctg
      541 gcgaccaacc cggaagcgca gcgcctgctg attgaaagcg gcggcgaaag cctgcgcgcg
      601 ggcgtgcgca acatgatgga agatctgacc cgcggcaaaa ttagccagac cgatgaaagc
      661 gcgtttgaag tgggccgcaa cgtggcggtg accgaaggcg cggtggtgtt tgaaaacgaa
      721 tattttcagc tgctgcagta taaaccgctg accgataaag tgcatgcgcg cccgctgctg
      781 atggtgccgc cgtgcattaa caaatattat attctggatc tgcagccgga aagcagcctg
      841 gtgcgccatg tggtggaaca gggccatacc gtgtttctgg tgagctggcg caacccggat
      901 gcgagcatgg cgggcagcac ctgggatgat tatattgaac atgcggcgat tcgcgcgatt
      961 gaagtggcgc gcgatattag cggccaggat aaaattaacg tgctgggctt ttgcgtgggc
     1021 ggcaccattg tgagcaccgc gctggcggtg ctggcggcgc gcggcgaaca tccggcggcg
     1081 agcgtgaccc tgctgaccac cctgctggat tttgcggata ccggcattct ggatgtgttt
     1141 gtggatgaag gccatgtgca gctgcgcgaa gcgaccctgg gcggcggcgc gggcgcgccg
     1201 tgcgcgctgc tgcgcggcct ggaactggcg aacaccttta gctttctgcg cccgaacgat
     1261 ctggtgtgga actatgtggt ggataactat ctgaaaggca acaccccggt gccgtttgat
     1321 ctgctgtttt ggaacggcga tgcgaccaac ctgccgggcc cgtggtattg ctggtatctg
     1381 cgccatacct atctgcagaa cgaactgaaa gtgccgggca aactgaccgt gtgcggcgtg
     1441 ccggtggatc tggcgagcat tgatgtgccg acctatattt atggcagccg cgaagatcat
     1501 attgtgccgt ggaccgcggc gtatgcgagc accgcgctgc tggcgaacaa actgcgcttt
     1561 gtgctgggcg cgagcggcca tattgcgggc gtgattaacc cgccggcgaa aaacaaacgc
     1621 agccattgga ccaacgatgc gctgccggaa agcccgcagc agtggctggc gggcgcgatt
     1681 gaacatcatg gcagctggtg gccggattgg accgcgtggc tggcgggcca ggcgggcgcg
     1741 aaacgcgcgg cgccggcgaa ctatggcaac gcgcgctatc gcgcgattga accggcgccg
     1801 ggccgctatg tgaaagcgaa agcgCATCAC CATCACCATC ATCACtaaCC AGGCATCAAA
     1861 TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA
     1921 ACGCTCTCTA CTAGAGTCAC ACTGGCTCAC CTTCGGGTGG GCCTTTCTGC GTTTATA
//