<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Week 6 HW: Genetic Circuits Part I: Assembly Technologies :: 2026a-justine-de-riedmatten</title><link>https://pages.htgaa.org/2026a/justine-de-riedmatten/homework/week-06-hw-genetic-circuits-part-i/index.html</link><description>Assignment: DNA Assembly 1. What are some components in the Phusion High-Fidelity PCR Master Mix, and what is their purpose?
Phusion High-Fidelity PCR Master Mix offers high fidelity and performance for PCR. It consists of Phusion DNA polymerase (1), deoxynucleotides and reaction buffer that has been optimised and also includes MgCl2 (New England Biolabs, 2026). The DNA Polymerase allows for the rapid synthesis of a new DNA strand with high accuracy, as it generates long templates with a single enzyme (Thermo Scientific, 2018). The deoxynucleotides are the essential building blocks for the DNA polymerase to proceed with the synthesis of a new DNA strand, whilst the buffer is what provides the ideal environment for the DNA polymerase to function, resulting in high-yield and fidelity.</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/justine-de-riedmatten/homework/week-06-hw-genetic-circuits-part-i/index.xml" rel="self" type="application/rss+xml"/></channel></rss>