https://pages.htgaa.org/2026a/justine-de-riedmatten/homework/week-09-hw-cell-free-systems/index.htmlHomework Part A: General and Lecturer-Specific Questions General homework questions 1. Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell-free expression is more beneficial than cell production. Cell-free protein synthesis provides greater flexibility and control compared to traditional in vivo expression, as the reaction occurs outside living cells, allowing factors such as DNA concentration and energy components to be adjusted without affecting cell viability. In in vivo expression methods, amplifying the bacterial plasmid, sequencing it, expressing the strain, and purifying the protein must be done separately for every single protein in the pathway. In contrast, in cell-free protein expression using a synthetic cell, proteins can be expressed directly from linear PCR fragments without the need for plasmid construction. This makes the process much more time‑efficient and allows results to be obtained within hours. Furthermore, all proteins can be expressed in one pot by adjusting the concentrations of the DNA, unlike in vivo systems that require different promoters for each protein and expression level.Hugoen