<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Week 6 HW: Genetic Circuits Part I: Assembly Technologies :: 2026a-karina-campos</title><link>https://pages.htgaa.org/2026a/karina-campos/homework/week-06-hw-genetic-circuits-part-i/index.html</link><description>Part A. DNA Assembly
What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Phusion High-Fidelity DNA Polymerase: A Pfu-like enzyme fused to a dsDNA-binding domain (Sso7d). This increases processivity and ensures an error rate 50 times lower than Taq polymerase. 5X Phusion HF Buffer (including $MgCl_2$): Maintains optimal pH and provides Magnesium ions, which act as essential cofactors for the polymerase to catalyze the addition of dNTPs. dNTPs (Deoxynucleotide Triphosphates): The molecular “bricks” (dATP, dTTP, dCTP, dGTP) used to synthesize the new DNA strand. Stabilizers: Often including glycerol or mild detergents to maintain enzyme stability through repeated thermal cycling.</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/karina-campos/homework/week-06-hw-genetic-circuits-part-i/index.xml" rel="self" type="application/rss+xml"/></channel></rss>