https://pages.htgaa.org/2026a/karina-campos/homework/week-10-hw-imaging-and-measurement/index.htmlWaters Part I — Molecular Weight Calculated Molecular Weight: Based on the provided 246 amino acid sequence (including the LE-linker and 6xHis-tag), the theoretical molecular weight is 27,845.03 Da. However, considering the maturation of the eGFP chromophore (cyclization and oxidation of the Thr65-Tyr66-Gly67 triad), there is a loss of 18 Da (for $H_2O$ loss) and 2 Da (for oxidation/dehydrogenation) in some variants. Therefore, the expected intact mass observed in the LC-MS would be approximately 27,825–27,827 Da.Hugoen