https://pages.htgaa.org/2026a/karina-campos/homework/week-11-hw-bioproduction/index.htmlPart A: The 1,536 Pixel Artwork Canvas | Collective Artwork Part B: Cell-Free Protein Synthesis | Cell-Free Reagents Each component’s role is in the cell-free reaction. E. coli Lysate (BL21 (DE3) Star): Provides the essential biological “hardware,” including ribosomes, tRNAs, and various translation factors; it specifically includes T7 RNA Polymerase to drive high-level transcription from T7 promoters. Salts and Buffers: Potassium Glutamate: Acts as the primary potassium source and a major intracellular salt, which is critical for maintaining proper osmotic pressure and supporting protein-DNA interactions during transcription and translation. HEPES-KOH pH 7.5: Serves as a chemical buffering agent to maintain a stable physiological pH throughout the reaction, preventing acidification from metabolic byproducts. Magnesium Glutamate: Provides essential $Mg^{2+}$ ions that act as necessary cofactors for ribosome assembly and the enzymatic activity of polymerases. Potassium Phosphate (Monobasic & Dibasic): Maintains phosphate homeostasis and contributes to pH stability, while also providing inorganic phosphate for nucleotide recycling. Energy and Nucleotide System:Hugoen