https://pages.htgaa.org/2026a/karina-frances-edmonds/homework/week-9-hw-cell-free-systems/index.html1. Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell-free expression is more beneficial than cell production. The first advantage of cell-free protein synthesis (CFPS) over traditional in vivo methods is that it avoids the ethical concerns associated with modifying living cells; CFPS uses cellular machinery such as ribosomes and enzymes. The second advantage of CFPS is the time and cost. CFPS takes around 1-2 hours while cell-based expressions, such as in E. coli, take around a few days to a week, depending on the expression. Third, there is increased biosafety and controllability with CFPS. CFPS are more controllable and programmable. Unlike using living cells which can escape into the environment and potentially cause harm depending on the organism. LastlyHugoen