https://pages.htgaa.org/2026a/karol-duque/homework/week-06-hw-genetic-circuits-part-i/index.htmlDNA Assembly What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Phusion HF PCR Master Mix is a 2X pre-formulated reagent containing several key components: Phusion DNA Polymerase: A thermostable, high-fidelity polymerase derived from Pyrococcus-like archaeal enzymes, fused to a processivity-enhancing DNA-binding domain (Sso7d). It synthesizes new DNA strands from dNTPs. 3’→5’ proofreading exonuclease: Excises misincorporated nucleotides immediately after insertion, giving Phusion an error rate ~50× lower than Taq polymerase Molecular Biology International. This is critical for mutagenesis work where a single wrong base would ruin the experiment.Hugoen