Abstract Phage satellites–mobile genetic elements which hijack a helper phage to propagate–have great potential to revolutionize synthetic biology applications. In particular, phage satellites have also been employed to deliver genetic circuits to the bacterial chromosome, including CRISPR/Cas9 cassettes that can target virulence genes to weaken pathogens (Ram et al 2018). A novel phage satellite class, Extracellular Prophage-Inducing Particles (EPIPs), can target Mycobacteriaceae and thus have particularly relevant applications for engineering native soil microbial communities to improve environmental health as well as treating common mammalian pathogens causing tuberculosis and leprosy (Qian et al., 2025). Mycobactericae can be particularly tricky to engineer using traditional plasmid techniques due to their mycolic acid walls preventing easy uptake of foreign DNA (Dao et al 2025). Already, mycobacteriophage-based DNA delivery has been found to increase transduction efficiency, including to deliver resolvases that knockout hygromycin antibiotic resistance in M. tuberculosis (Jain et al 2014), and other phage satellite classes infecting different bacterial hosts (such as SaPIs) can deliver circuits in a similar infection and integration manner (Ram et al 2018). These phage and phage-satellite methods are also advantageous in that modifications do not require antibiotic selection to persist, which is important given the rising biosafety concerns of horizontal gene transfer of antibiotic resistance (de Lorenzo and Martinez-Garcia 2025) particularly given a common antibiotic resistance cassette used in plasmid engineering, kanamycin, also confers resistance to amino glycides, a drug class used to treat tuberculosis infections (Yang et al 2015). I thus propose to modify one such EPIP capable of integrating into the M. aichiense genome, the EPIP named Bernie, to investigate its potential use as a microbial community editor. Specificially, I will use Golden Gate Assembly to re-assemble Bernie with the red fluorescence protein mCherry and the strong psmyc promoter optimized for myco, so that engineered Bernie DNA could be electroporated into the M. aichiense host, the phage satellite could reboot and infect, and the bacterial host would then express the red fluorescence gene newly integrated into its genome (as assessed using plate reader techniques).